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ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-16      瀏覽次數(shù):232

ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒


Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類多樣,操作簡(jiǎn)單,靈敏度高。

認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

Arbor Assays公司的主要產(chǎn)品包括:

DetectX® Kits

Inhibitors & Activators

AbX® Antibodies & Affinity Resins

Detection Systems

Components & Ancillary Reagents

所需材料:

類固醇標(biāo)準(zhǔn)品,用于檢測(cè)萃取效率

ACS級(jí)乙醇(或乙酸乙酯)

玻璃試管

處理流程:

確保樣品wan全干燥,并對(duì)樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測(cè)結(jié)果來確定提取效率,詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過被動(dòng)干燥、溫和加熱(60°C)或冷凍的方式來進(jìn)行干燥處理。

2.0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

3.用力搖晃至少30分鐘。

4.4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

注:含有低水平分析物的樣品可以通過干燥提取液和在少量分析緩沖液中重懸,來達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存。或用乙醇(100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

 5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μLAB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析,測(cè)定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測(cè)緩沖液進(jìn)行稀釋。

6.根據(jù)試劑盒說明書對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

 

下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

產(chǎn)品名稱

貨號(hào)

規(guī)格

Goat Anti-Mouse IgG Fc, 25MG

A008-25MG

25MG

Cyclic AMP Direct ELISA Kit (1 Plate)

K019-H1

1x96 well plate

Corticosterone ELISA Kit (1 Plate)

K014-H1

1x96 well plate

Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

K027-H1

1x96 well plate

Aldosterone ELISA Kit (1 Plate)

K052-H1

1x96 well plate

Urea Nitrogen (BUN) Detection Kit (2 Plate)

K024-H1

2x96 well plate

Cortisol ELISA Kit (1 Strip plate)

K003-H1

1x96 well plate

PGFM ELISA Kit (1 Plate)

K022-H1

1x96 well plate

Triiodothyronine (T3) ELISA Kit (1 Plate)

K056-H1

1 Kit

FRAP™ (Ferric Reducing Antioxidant Power) Detection   Kit (2 Plate)

K043-H1

2x96 well plate

Hemoglobin Detection Reagent, 20ML

C038-20ML

20ML

Ceruloplasmin Colorimetric Activity Kit (2 Plate)

K035-H1

2x96 well plate

ThioStar™ Fluorescent Thiol Substrate, 100UG

L002-100UG

100UG

Cyclic GMP Direct Chemiluminescent ELISA Kit (1   Plate)

K020-C1

1x96 well plate

Progesterone ELISA Kit (1 Plate)

K025-H1

1x96 well plate

Testosterone ELISA Kit (1 Plate)

K032-H1

1x96 well plate

Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

K036-H1

1x96 well plate

Thyroxine (T4) ELISA Kit (1 Plate)

K050-H1

1x96 well plate

Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1   Plate)

K051-H1

1x96 well plate

Tranylcypromine

X042-1EA

1EA

Triiodothyronine (T3) ELISA Kit (5 Plate)

K056-H5

5x96 well plate

Glutathione Colorimetric Detection Kit (4 Plate)

K006-H1

4x96 well plate

Cyclic GMP Direct ELISA Kit (1 Plate)

K020-H1

1x96 well plate

Progesterone ELISA Kit (5 Plate)

K025-H5

5x96 well plate

Cyclic AMP Direct ELISA Kit (5 Plate)

K019-H5

5x96 well plate

Estradiol ELISA Kit (1 Plate)

K030-H1

1x96 well plate

Oxytocin ELISA Kit (1 Plate)

K048-H1

1x96 well plate

Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

K036-H5

5x96 well plate

Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5   Plate)

K037-H5

5x96 well plate

Hemoglobin Standard, 300UL

C037-300UL

300UL

DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

C133-3ML

3ML

Creatinine Urinary Detection Kit (2 Plate)

K002-H1

2x96 well plate

Butyrylcholinesterase Fluorescent Activity Kit (2   Plate)

K016-F1

2x96 well plate

Corticosterone ELISA Kit, (5 Plate)

K014-H5

5x96 well plate

Cyclic GMP Direct Chemiluminescent ELISA Kit (5   Plate)

K020-C5

5x96 well plate

Superoxide Dismutase (SOD) Colorimetric Activity   Kit (2 Plate)

K028-H1

2x96 well plate

Catalase Colorimetric Activity Kit (2 Plate)

K033-H1

2x96 well plate

Glutathione Fluorescent Detection Kit (1 Plate)

K006-F1

1x96 well plate

Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

C128-625UL

625UL

Thyroxine (T4) ELISA Kit (5 Plate)

K050-H5

5x96 well plate

Acetylcholinesterase Fluorescent Activity Kit (2   Plate)

K015-F1

2x96 well plate

Creatinine Serum Kit (2 Plate)

KB02-H1

2x96 well plate

Hydrogen Peroxide Fluorescent Detection Kit (2   Plate)

K034-F1

2x96 well plate

Creatinine Serum Low Sample Volume Kit (384-well   Plate)

KB02-H1D

1x384 well plate

Recombinant Obelin, 100UG

L001-100UG

100UG

Dry DMSO, 20ML

X022-20ML

20ML

Dry DMSO, 2ML

X022-2ML

2ML

Urea Nitrogen Standard, 1ML

C089-1ML

1ML

NADPH lyophilized, 1EA

X045-1EA

1EA

DetectX® PGE2 Conjugate, 13ML

C179-13ML

13ML

17-Hydroxyprogesterone   ELISA Kits

K053-H

1 Kit

Arg8-Vasopressin (AVP) ELISA Kits

K049-H

1 Kit

Arg8-Vasopressin (AVP) Chemiluminescent   ELISA Kits

K049-C

1 Kit

Androstenedione ELISA Kits

K070-H

1 Kit

Aldosterone Chemiluminescent ELISA Kits

K052-C

1 Kit

Allopregnanolone ELISA Kits

K061-H

1 Kit

Adrenocorticotropic Hormone (ACTH) ELISA   Kits

K072-H

1 Kit

Alkaline Phosphatase (ALP) Colorimetric   Activity Kit

K082-H1

1 Kit

3,3-Cyclic GAMP ELISA Kits

K073-H

1 Kit

20-Hydroxyecdysone ELISA Kit

K066-H

1 Kit

2,3-Cyclic GAMP STING-Based FRET Detection Kits

K081-F

1 Kit

2,3-Cyclic GAMP ELISA Kits

K067-H

1 Kit

Cyclic GMP Direct ELISA Kits – Improved   Sensitivity

K065-H

1 Kit

Endothelin-1 (ET-1) ELISA Kit

K045-H1

1 Kit

Estrone-3-Sulfate (E1S) ELISA Kits

K038-H

1 Kit

Assay Buffer, 5X

X065-110ml

110ml

Blocking Buffer, 10X

X109-25ml

25ml

Goat anti-Rabbit IgG Coated Plate

X016-1EA


HRP Conjugate Diluent

X076-300ml

300ml

TMB Substrate

X019-55ml

55ml

Mesotocin Solution (500ng/mL)

X127-625UL


5-Azacytidine

P012-50MG

50mg

 

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