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Epigentek 創(chuàng)新技術(shù)和表觀遺傳學(xué)-相關(guān)技術(shù)文章

更新時間:2024-07-17      瀏覽次數(shù):229

Epigentek 創(chuàng)新技術(shù)和表觀遺傳學(xué)

Epigentek集團(tuán)公司是全球創(chuàng)新技術(shù)和表觀遺傳學(xué)相關(guān)研究產(chǎn)品的開發(fā)商和供應(yīng)商。公司開發(fā)了包括400多個產(chǎn)品的全面的產(chǎn)品組合,為表觀遺傳學(xué)方面的研究和新藥研發(fā)提供全面系統(tǒng)的解決方案。2019年,Epigentek榮獲“Bioz Rising Star TM ",被評為2019表觀遺傳學(xué)領(lǐng)域的新星公司。

Epigentek公司的產(chǎn)品銷售領(lǐng)域

全shi界的備受推崇的學(xué)者、制藥企業(yè)以及很多專業(yè)的生物技術(shù)公司都在使用Epigentek產(chǎn)品。相比普通方法的產(chǎn)品而言,這些du特的產(chǎn)品在科研分析過程中更簡單、更快捷、更方便、更有效。Epigentek公司的產(chǎn)品通過專門的銷售隊伍和遍布全球超過36個國家和地區(qū)的經(jīng)銷商進(jìn)行銷售。讓客戶滿意和幫助客戶在試驗中取得最大成功是我們進(jìn)行技術(shù)創(chuàng)新的首要任務(wù)。公司特別強(qiáng)調(diào)技術(shù)的速度和效率,繼續(xù)側(cè)重于表觀遺傳學(xué)創(chuàng)新和廣泛發(fā)展,提高其產(chǎn)品組合。

Epigentek公司的產(chǎn)品類型

專業(yè)提供DNA甲基化試劑盒、組蛋白修飾相關(guān)試劑盒、染色質(zhì)免疫沉淀反應(yīng)試劑盒、基因表達(dá)與沉默檢測試劑盒、DNA損傷及修復(fù)試劑盒、SUMO化修飾分析試劑盒、表型相關(guān)抗體等表觀遺傳學(xué)產(chǎn)品。

蛋白質(zhì)與DNA之間的動態(tài)相互作用,參與并推動了基因的表達(dá)、調(diào)控、復(fù)制、重組和修復(fù)以及RNA的轉(zhuǎn)運(yùn)、翻譯和調(diào)控等多個生物學(xué)過程,幾乎涉及所有的生命活動。繪制這些蛋白質(zhì)-DNA結(jié)合事件發(fā)生的時空關(guān)系,對于正常發(fā)育和疾病中表觀遺傳學(xué)的機(jī)理研究至關(guān)重要。

為了生成這種數(shù)據(jù),科學(xué)家通常使用ChIP(染色質(zhì)免疫沉淀)-Seq法。傳統(tǒng)的ChIP-Seq實(shí)驗需要花費(fèi)三天來完成:首先進(jìn)行染色質(zhì)交聯(lián)(使用甲醛作為交聯(lián)劑),使基因組上分布的蛋白以及各種修飾定格下來,然后對交聯(lián)的染色質(zhì)進(jìn)行超聲裂解,再利用抗原抗體特異性反應(yīng)將與目的蛋白相結(jié)合的DNA片段沉淀下來,再進(jìn)行解交聯(lián)釋放結(jié)合蛋白的DNA片段,最后對獲得的DNA片段進(jìn)行測序分析。這些繁瑣的步驟中會大量損失樣本和信息,因此傳統(tǒng)的ChIP-Seq方法具有需要大量細(xì)胞、重復(fù)性差、低信號、高背景等缺點(diǎn)。

2017年,Henikoff博士及其實(shí)驗室的研究人員在eLife雜志上發(fā)表了一種稱為CUT&RUN的新方法。CUT&RUN(Cleavage Under Targets and Release Using),即核酸酶靶向切割及釋放技術(shù)。CUT&RUN技術(shù)的出現(xiàn),克服了傳統(tǒng)ChIP-seq方法的缺陷,可以在細(xì)胞天然染色質(zhì)環(huán)境下進(jìn)行蛋白-DNA互作的研究。

Epigentek公司的熱銷產(chǎn)品

產(chǎn)品名稱

貨號

規(guī)格

MethylFlash Methylated DNA 5-mC   Quantification Kit (Colorimetric)

P-1034-96

96 Assays

EpiQuik Total Histone   Extraction Kit

OP-0006-100

100 Extractions

EpiQuik Nuclear Extraction Kit

OP-0002-1

100 Assays

BisulFlash DNA Modification Kit

P-1026-050

50 Reactions

EpiQuik DNA Methyltransferase   (DNMT) Activity/Inhibition Assay Kit

P-3001-1

48 Assays

MethylFlash Methylated DNA 5-mC   Quantification Kit (Colorimetric)

P-1034-48

48 Assays

EpiQuik m6A RNA Methylation   Quantification Kit (Colorimetric)

P-9005-96

96 Reactions

EpiQuik m6A RNA Methylation   Quantification Kit (Colorimetric)

P-9005-48

48 Reactions

MethylFlash Hydroxymethylated   DNA 5-hmC Quantification Kit (Colorimetric)

P-1036-96

96 Assays

EpiQuik Chromatin   Immunoprecipitation (ChIP) Kit

P-2002-1

24 Reactions

Methylamp DNA Modification Kit

P-1001-2

80 Reactions

MethylFlash Global DNA   Methylation (5-mC) ELISA Easy Kit (Colorimetric)

P-1030-96

96 reactions

MethylFlash Methylated DNA   Quantification Kit (Fluorometric)

P-1035-96

96 Assays

EpiQuik Plant ChIP Kit

P-2014-48

48 Reactions

5-Methylcytosine (5-mC)   Monoclonal Antibody [33D3]

A-1014-050

50 ug

Methylamp Global DNA   Methylation Quantification Ultra Kit

P-1014B-48

48 Assays

Methylamp DNA Modification Kit

P-1001-1

40 Reactions

Methylamp Global DNA   Methylation Quantification Ultra Kit

P-1014B-96

96 Assays

EpiQuik DNMT   Activity/Inhibition Assay Ultra Kit (Colorimetric)

P-3009-48

48 Assays

EpiQuik Plant ChIP Kit

P-2014-24

24 Reactions

MethylFlash Hydroxymethylated   DNA 5-hmC Quantification Kit (Colorimetric)

P-1036-48

48 Assays

Methylamp Methylated DNA   Capture (MeDIP) Kit

P-1015-48

48 Reactions

EpiQuik 8-OHdG DNA Damage   Quantification Direct Kit (Colorimetric)

P-6003-96

96 Assays

Epigenase HDAC   Activity/Inhibition Direct Assay Kit (Colorimetric)

P-4034-96

96 Assays

EpiQuik Tissue Chromatin   Immunoprecipitation (ChIP) Kit

P-2003-1

24 Reactions

MethylFlash Hydroxymethylated   DNA Quantification Kit (Fluorometric)

P-1037-96

96 Assays

MethylFlash Methylated DNA   Quantification Kit (Fluorometric)

P-1035-48

48 Assays

EpiQuik Total Histone H3   Acetylation Detection Fast Kit (Colorimetric)

P-4030-96

96 Assays

Methylamp Whole Cell Bisulfite   Modification Kit

P-1016-80

80 Samples

EpiQuik Total Histone H4   Acetylation Detection Fast Kit (Colorimetric)

P-4032-96

96 Assays

EpiQuik DNA Methyltransferase   (DNMT) Activity/Inhibition Assay Kit

P-3001-2

96 Assays

EpiQuik Global Histone H3   Acetylation Assay Kit

P-4008-96

96 Assays

Histone H3K9me3 (H3K9   Trimethyl) Polyclonal Antibody

A-4036-025

25 ul

EpiQuik Chromatin   Immunoprecipitation (ChIP) Kit

P-2002-2

48 Reactions

EpiQuik HDAC   Activity/Inhibition Assay Kit (Colorimetric)

P-4002-96

96 Assays

EpiQuik MeDIP Ultra Kit

P-1052-48

48 Reactions

5-Methylcytosine (5-mC)   Monoclonal Antibody [33D3]

A-1014-010

10 ug

EpiQuik DNMT   Activity/Inhibition Assay Ultra Kit (Colorimetric)

P-3009-96

96 Assays

EpiQuik Hydroxymethylated DNA   Immunoprecipitation (hMeDIP) Kit

P-1038-24

24 Reactions

EpiQuik Global Histone H4   Acetylation Assay Kit

P-4009-96

96 Assays

EpiQuik One-Step DNA Hydrolysis   Kit

P-1023-96

96 Samples

EpiQuik Global Histone H3   Acetylation Assay Kit

P-4008-48

48 Assays

EpiQuik Global Histone H4   Acetylation Assay Kit

P-4009-48

48 Assays

5-Methylcytosine (5-mC)   Monoclonal Antibody [33D3]

A-1014-100

100 ug

Histone H3K9me2 (H3K9 Dimethyl)   Polyclonal Antibody

A-4035-050

50 ul

Histone H3K27me3 (H3K27   Trimethyl) Polyclonal Antibody

A-4039-025

25 ul

EpiQuik Tissue Acetyl-Histone   H3 ChIP Kit

P-2012-24

24 Reactions

EpiQuik Dnmt1 Assay Kit

P-3011-2

48 Assays

EpiQuik HAT Activity/Inhibition   Assay Kit

P-4003-96

96 Assays

EpiQuik Tissue Chromatin   Immunoprecipitation (ChIP) Kit

P-2003-2

48 Reactions



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